Genetic Diversity of Dodder (cuscuta Spp.) Collected from Khartoum and Gezira States

The study was carried out at the field dodder (Cuscuta spp.) in Khartoum and Gezira states, to study variation between 28 samples of it in two States using DNA Markers (RAPD). Four primers were used (OPAL15, OPAL18, OPB17 and OPAL20) to amplify the genomic DNA of 28 samples of Cuscuta spp. Two primers (OPB17, OP AL20) showed high percentage (100%) to amplify the genomic DNA polymorphism bands, while the other two primers (OPAL15, OPAL18) were produced (91.7%) polymorphism bands. UPGA analysis showed RAPD Distance Matrix range (0.10-0.98) which reflected high genetic diversity. The RAPD UPGA Tree Diagram showed high Molecular Variation within States. The resulting of four RAPD Markers analyses were Observed that tree diagram showed two groups A and B . Group A Consists of two sub group. As sub –group A1, and sub-group A2. Sub –group A1 have two sisters (JebalRamtook and Jebal-Molokha) ,(Toti-Remet and JebalAmoyogha ) and sub-group A2 have five sisters (Alkabash Alfalfa and Alfaki-hashimAlfalfa) ,(Wad-AlkawahalaOnion and wada-Almajzoub Onion ),(Shambat-Lime and Shambat Balloon plant),(Alalaphon Alfalfa and Omdurmanlime ) and ( Gezira Slang Alfalfa and Shambat Alfalfa).A group A and B correlated Consists variables. group B Have one sisters (HantoobOnion and Madina-Arab, Dahaser), (Helat-kokGhobash) As Subgroup A1, (Algeli Alfalfa, TotiAlfalfa, KhartoumDames, wad-Alkawahala-Onion and Algeli Ramtook) has sub-group A2, (Shambat –Euphorbia ) correlate with sub-A1, And A2 was showed sample (Alalaphon Dahaser) as out group and genetically close to each other’s (sisters).