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Quantification of the Number of Adsorbed DNA Molecules on Single-Walled Carbon Nanotubes

Journal of Physical Chemistry C(2019)SCI 3区

Gottingen Univ | Univ Texas El Paso

Cited 59|Views25
Abstract
Single-walled carbon nanotubes (SWCNTs) have unique photophysical properties and promise many novel applications. Noncovalent functionalization with single-stranded DNA (ssDNA) is one of the most used approaches and variation of ssDNA sequences lead to major advances in separation of SWCNT chiralities and SWCNT-based sensors. However, the exact number of adsorbed ssDNA molecules on ssDNA/SWCNT complexes vary in literature and are overall not precisely known. Here, we determine the number of adsorbed ssDNA molecules per SWCNT for different ssDNA sequences. For this purpose, we directly quantified free and adsorbed ssDNA and the concentration of SWCNTs using an approach based on filtration, absorption spectroscopy, and atomic force microscopy. The number of adsorbed ssDNA molecules on 600 nm long (6,5)-SWCNTs varied between ∼860 for (GT) 5 and ∼130 for (A) 30 . The sequence (GT) 15 occupied an average SWCNT segment of ∼2.3 nm, while the SWCNT segment length of (GT) x repeats increased linear with sequence length x. Molecular dynamics simulations with experimentally determined values as parameters showed that (GT) 15 ssDNA stacked on top of each other on the SWCNTs in contrast to simulations with an excess SWCNT surface. By knowing the number of adsorbed ssDNA molecules per SWCNT it is further more possible to address ratio-specific DNA conjugation approaches. In summary, these results provide novel quantitative insights into the complex structure of DNA/SWCNT hybrids.
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Single-Molecule Sensing,DNA nanotechnology,DNA Sequencing
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要点】:本文通过实验方法精确测定了不同序列的单链DNA在单壁碳纳米管上的吸附数量,为DNA/SWCNT复合物的结构研究提供了定量数据。

方法】:采用过滤、吸收光谱和原子力显微镜技术直接量化了自由和吸附的ssDNA以及SWCNT的浓度。

实验】:通过实验确定了不同长度和序列的ssDNA在SWCNT上的吸附数量,例如(GT) 5序列的吸附数量约为860个分子,(A) 30序列的吸附数量约为130个分子,并使用分子动力学模拟验证了实验结果。