Electrografting of diazonium salt for SPR application

A general method to develop diazonium-based biochip for SPRi analysis is presented. The electrografting of carboxybenzene diazonium salt is optimized in order to fit with the requirement of the SPR technology. The influence of the surface preparation on plasmon quality is discussed and the performance of this original modified biochip is investigated for determining the binding constants between circulating ovalbumin and spotted anti-ovalbumin antibody. The kinetic constants are calculated and compared with literature and similar performances are reached confirming that diazonium based biochip is a good complement to commercial SPRi sensor.