Loss of microbial diversity and body site heterogeneity in individuals with Hidradenitis Suppurativa
Journal of Investigative Dermatology(2019)
摘要
Hidradenitis Suppurativa (HS) is a chronic, scarring, inflammatory skin disease affecting hair follicles in axillae, inguinal, and anogenital regions. Dysbiosis in HS patients compared to healthy subjects is documented. However, whether dysbiosis is specific to particular body sites or skin niches is unknown. We investigated the follicular and skin surface microbiome of the axilla and groin of HS patients (n=11) and healthy individuals (n=10) using 16S rRNA gene sequencing (V3-V4). We sampled non-lesional (HSN) and lesional skin (HSL) of HS patients. β-diversity was significantly decreased (p<0.05) in HSN and HSL skin compared to normal skin with loss of body site and skin niche heterogeneity in HS samples. The relative bacterial abundance of specific microbes was also significantly different between normal and HSN (15 genera) or HSL (21 genera) skin. Smoking and alcohol use influenced the β-diversity (p<0.08) in HS skin. We investigated metabolic profiles of bacterial communities in HS and normal skin using a computational approach. Metabolism, Genetic Information Processing , and Environmental Information Processing were significantly different between normal and HS samples. Altered metabolic pathways associated with dysbiosis of HS skin suggest mechanisms underlying the disease pathology and information about treatment with drugs targeting those pathways.
* FWER
: family-wise error rate
HS
: Hidradenitis Suppurativa
HSN
: HS non-lesional
HSL
: HS lesional
KEGG
: Kyoto Encyclopedia of Genes and Genomes
MCC
: mock-community control
MiRKAT
: Microbiome Regression Kernel Association Test
OTUs
: operational taxonomic units
PICRUSt
: Phylogenetic Investigation of Communities by Reconstruction of Unobserved States
PSU
: pilosebaceous unit
STAMP
: Statistical Analysis of Metagenomic Profile
更多查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要