In vitro probiotic properties of selected lactobacilli and multi-strain consortium on immune function, gut barrier strengthening and gut hormone secretion

•Three Lactobacillus strains from vaginal flora were studied for their probiotic traits.•The strain 395 and the 3RG consortium exhibit anti-inflammatory effect on PBMC model.•L. gasseri ICVB392 and the 3RG consortium strengthen Caco-2 epithelial barrier.•The lactobacilli strains are able to modulate the secretion of CCK and GLP-1 hormones.•Specific antagonist/inhibitor impact differentially the secretion of these hormones.