MP70-06 PREOPERATIVE ANTIBODY TITER AGAINST BK POLYOMAVIRUS MAY PREDICT BK POLYOMAVIRUS-ASSOCIATED NEPHROPATHY AFTER KIDNEY TRANSPLANTATION

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery II (MP70)1 Apr 2019MP70-06 PREOPERATIVE ANTIBODY TITER AGAINST BK POLYOMAVIRUS MAY PREDICT BK POLYOMAVIRUS-ASSOCIATED NEPHROPATHY AFTER KIDNEY TRANSPLANTATION Mitsuru Saito*, Shigeru Satoh, Takuro Saito, Ryohei Yamamoto, Taketoshi Nara, Syuji Chiba, Sohei Kanda, Kazuyuki Numakura, Shintaro Narita, Takamitsu Inoue, and Tomonori Habuchi Mitsuru Saito*Mitsuru Saito* More articles by this author , Shigeru SatohShigeru Satoh More articles by this author , Takuro SaitoTakuro Saito More articles by this author , Ryohei YamamotoRyohei Yamamoto More articles by this author , Taketoshi NaraTaketoshi Nara More articles by this author , Syuji ChibaSyuji Chiba More articles by this author , Sohei KandaSohei Kanda More articles by this author , Kazuyuki NumakuraKazuyuki Numakura More articles by this author , Shintaro NaritaShintaro Narita More articles by this author , Takamitsu InoueTakamitsu Inoue More articles by this author , and Tomonori HabuchiTomonori Habuchi More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000557098.44822.41AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: BK polyomavirus (BKPyV) is a causative agent of BKPyV-associated nephropathy (BKPyVAN) and hemorrhagic cystitis, which occurs subsequent to BKPyV reactivation. Intravenous immunoglobulin is presumed to have significant neutralizing activity in vitro; therefore, a high-antibody titer against BKPyV should be effective in controlling BKPyV-associated diseases. This study aimed to measure the preoperative anti-BKPyV antibody titer in both kidney transplant recipients and living donors and investigate the significance of a preoperative anti-BKPyV antibody titer as a clinical marker. METHODS: From July 2013 to December 2016, 68 consecutive kidney living-donor-recipient pairs underwent kidney transplantation at Akita University Hospital. First, we obtained urine and blood samples of the kidney transplant recipients before and 1, 3, 6, and 12 months after kidney transplantation. Next, we measured the DNA copy number of BKPyV by quantitative real-time polymerase chain reaction. Anti-BKPyV antibody titers of the serum of both recipients and living donors were evaluated preoperatively by neutralizing activity using enzyme-linked immunosorbent assay. RESULTS: In this study, nine kidney transplant recipients (14.3%) developed viremia and three (4.8%) developed BKPyVAN. Almost all the BKPyV-DNA subtypes among the detectable specimens were I/c (94.4%), which is the major subtype in Japan. In patients with BKPyVAN, we detected serum BKPyV-DNA by monitoring BKPyV-DNA several months before the patients developed symptomatic BKPyVAN. Multivariate analysis revealed that potent immunosuppressive protocol (odds ratio [OR] = 12.7; p = 0.044) and non-high titer of anti-BKPyV antibody of recipients or living donors (OR = 11.5; p = 0.049) were significant risk factors of viremia and/or BKPyVAN. CONCLUSIONS: Anti-BKPyV antibody titers of kidney transplant recipients and living donors may be a useful clinical marker for the potential risk of BKPyVAN. Source of Funding: none Akita, Japan© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e1039-e1040 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Mitsuru Saito* More articles by this author Shigeru Satoh More articles by this author Takuro Saito More articles by this author Ryohei Yamamoto More articles by this author Taketoshi Nara More articles by this author Syuji Chiba More articles by this author Sohei Kanda More articles by this author Kazuyuki Numakura More articles by this author Shintaro Narita More articles by this author Takamitsu Inoue More articles by this author Tomonori Habuchi More articles by this author Expand All Advertisement PDF downloadLoading ...