Metacommunity ecology meets bioassessment: Assessing spatio-temporal variation in multiple facets of macroinvertebrate diversity in human-influenced large lakes

•We examined drivers of 6 biotic metrics in a metacommunity context in two large lakes.•The relative roles of spatial, human-induced and natural factors were quantified.•The roles of spatial variables are comparable with local environmental conditions.•The relative roles of different drivers varied substantially among seasons.•Spatial processes, natural conditions and temporal variation should be considered.