Statistical and sequential (fill-and-draw) approach to enhance rhamnolipid production using industrial lignocellulosic hydrolysate C 6 stream from Achromobacter sp. (PS1).

•Cost effective rhamnolipid production using lignocellulosic hydrolysate.•Statistical optimization by response surface methodology.•Approx.1.65 fold increase in rhamnolipid yield.•Adoption of sequential fill-and-draw approach to further improve rhamnolipid yield.•Achievement of overall 19.35 g/L rhamnolipid yield using sequential approach.