A novel protein purification strategy mediated by the combination of CipA and Ssp DnaB intein.

•A novel protein purification method was proposed.•The method relied on a self-assembly protein CipA and a self-cleaving intein DnaB.•The purification process only needed centrifugation and cleavage steps.•Optimization of linker was carried out to enhance the purification efficiency.•MBP, Kivd and AdhP were also purified to broaden the application of this method.