Control of Gram-negative multi-drug resistant microorganisms in an Italian ICU: Rapid decline as a result of a multifaceted intervention, including conservative use of antibiotics.

•A multifaceted intervention to control selection and transmission of MDROs in the ICU.•An in-ward leader revised antibiotic prescription and involved all staff members.•ASP intervention included introduction of round-the-clock microbiological support.•Rapid and steep fall of Gram-negative resistant isolates after bundle implementation.