Defining threshold values in microscopic examination and qPCR for optimal performance of line probe assays for resistance detection in Mycobacterium tuberculosis complex.

Karoline Wagner, Benjamin Preiswerk,Peter M Keller,Bettina Schulthess

Diagnostic Microbiology and Infectious Disease（2019）

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摘要

Line probe assays enable rapid detection of drug-resistant Mycobacterium tuberculosis directly from clinical specimens. This study shows that defining threshold values in microscopic examination or PCR detection of M. tuberculosis optimizes line probe assay efficiency, thereby avoiding costs and loss of time due to unnecessary molecular testing.

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关键词

Mycobacterium tuberculosis,Line probe assay,qPCR,Microscopy,Drug resistance detection

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