iHi-C 2.0: a simple approach for mapping native spatial chromatin organisation from low cell numbers.

Athanasia Mizi,Eduardo Gade Gusmao,Argyris Papantonis

Methods（2020）

引用 6|浏览15

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摘要

•iHi-C is a crosslinking-free method for interrogating whole-genome spatial interactions.•The iHi-C 2.0 adaptation of the protocol is streamlined and requires minimal hands-on time.•Replacing blunt- with cohesive-end ligation increases efficiency.•iHi-C 2.0 maps from 105 human cells are rich in focal interactions.

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关键词

Chromosome conformation capture,Chromatin loop,Crosslinking-free,Massively-parallel sequencing,Topologically-associating domains,Nuclear architecture

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