Heteronuclear NMR spectroscopy of proteins encapsulated in cubic phase lipids.

•Successful encapsulation of 15N-enriched proteins in lipidic cubic phase.•No significant structural changes for LPC encapsulation were observed.•Reduced chemical exchange of backbone amides with solvent water was confirmed.•Additional sensitivity gain with the use of BEST RF sequences was demonstrated.•LCP encapsulation provides a novel means for quantifying residue-specific protein hydration.