Fast, Sensitive And Specific Detection Of Thailand Orthohantavirus And Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay
VIRUSES-BASEL(2019)
摘要
Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.
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关键词
Hantavirus, Thailand orthohantavirus, Real-time RT-PCR
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