Gene expression profiles of antigenic proteins of third stage larvae of the zoonotic nematode Anisakis pegreffii in response to temperature conditions.

Anisakis pegreffii, a recognised etiological agent of human anisakiasis, is a parasite of homeothermic hosts at the adult stage and of ectothermic hosts at the third larval stage. Among distinct factors, temperature appears to be crucial in affecting parasite hatching, moulting and to modulate parasite-host interaction. In the present study, we investigated the gene transcripts of proteins having an antigenic role among excretory secretory products (ESPs) (i.e., a Kunitz-type trypsin inhibitor, A. peg-1; a glycoprotein, A. peg-7; and the myoglobin, A. peg-13) after 24 h, in A. pegreffii larvae maintained in vitro, under controlled temperature conditions. Temperatures were 37 degrees C and 20 degrees C, resembling respectively homeothermic and ectothermic hosts conditions, and 7 degrees C, the cold stress condition post mortem of the fish host. Primers of genes coding for these ESPs to be used in quantitative real-time PCR were newly designed, and qRT-PCR conditions developed. Expression profiles of the genes A. peg-1 and A. peg-13 were significantly up-regulated at 20 degrees C and 37 degrees C, with respect to the control (larvae kept at 2 degrees C for 24 h). Conversely, transcript profiles of A. peg-7 did not significantly change among the chosen temperature conditions. In accordance with the observed transcript profiles, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of the three target ESPs at 37 degrees C, while only A. peg-13 was observed at 7 degrees C. The results suggest that temperature conditions do regulate the gene expression profiles of A. peg-1 and A. peg-13 in A. pegreffii larvae. However, regulation of the glycoprotein A. peg-7 is likely to be related to other factors such as the host's immune response.