Complete genomic sequence of Pseudomonas lactis bacteriophage HU1 isolated from raw cow’s milk

The lytic cold-active phage HU1, a member of the family Podoviridae , infects Pseudomonas lactis and was first isolated from raw cow’s milk. In this study, we used deep sequencing to determine and analyze the DNA genome sequence of HU1. We identified a 42,551-base-pair genome comprising double-stranded DNA, with 69 predicted open reading frames and a GC content of 56.4%. A whole-genome comparison did not identify HU1 as a member of any previously reported cluster of Pseudomonas phages. By contrast, HU1 was most similar to AF, which infects P. putida , with nucleotide sequence alignment coverage of 24%. These results suggest that HU1 is a novel Pseudomonas phage.