Development of an ultrasensitive label-free immunosensor for fungal aflatoxin B1 detection

•Development of a sensor allowing reliable aflatoxin B1 with very low LOD.•Cleaning process of SPEs permit to activate more sites and enhance their response.•Evaluation of three physicochemical treatment techniques for SA/antiAFB1 fixation.•Miniaturization of microsensor to strongly decrease the cost of the experiments.•Streptavidin-Biotin couple enhanced the amperometric response of the detection.