Quantitative analysis of underivatized 17 β-estradiol using a high-throughput LC-MS/MS assay - Application to support a pharmacokinetic study in ovariectomized guinea pigs.

•A sensitive and high-throughput LC-MS/MS assay for estradiol quantification in guinea pig plasma was validated.•The assay is novel, specific, robust, and reproducible for high-throughput settings.•The assay was used to profile estradiol pharmacokinetics in ovariectomized guinea pigs receiving exogenously administered estradiol.•In future, the assay could be used to monitor and compare endogenous estradiol levels in naïve guinea pigs with natural menstrual cycle.