ATP Synthase: Expression, Purification, and Function.

Meghna Sobti,Robert Ishmukhametov,Alastair G Stewart

PROTEIN NANOTECHNOLOGY: PROTOCOLS, INSTRUMENTATION, AND APPLICATIONS, 3RD EDITION（2020）

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摘要

ATP synthase is an essential enzyme found in all known forms of life, generating the majority of cellular energy via a rotary catalytic mechanism. Here, we describe the in-depth methods for expression, purification, and functional assessment of E. coli ATP synthase.

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关键词

ATP synthase,ATPase,Bioenergetics,Enzyme,Rotary motor

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