Raman Shift Characteristics of Substance Expression and Accumulation Structure in Lung Cancer Cells

To lay a foundation for the non-invasive examination of tumor cells, the associations between Raman shift characteristics and morphological parameters of cytochemical staining are analyzed in lung adenocarcinoma cells and physiological mesothelial cells. A549 cells and Met-5A cells arc analyzed using Raman spectroscopy to calculate the Raman spectral shift characteristics. A549 cells and Met-5A cells arc stained with H&E and PAP solutions to calculate their morphological image characteristics. In both cell lines, Raman shifts arc observed at 1082, 1155, and 1304 cm(-1). Energy standard deviations of A549 and Met-5A cells arc recognized by the images. PAP staining results arc significantly different (p <0.05), but H&E staining results show no significant differences (p >0.05) between the cell lines. The mean color value of cell image is significantly different (p <0. 05) between the two stains. The Raman shift characteristics of lung cancer cells arc then analyzed in terms of tumor substance expression and accumulation structure based on the affinity characteristics of the chemical substances. Experimental results show that clinical histopathological staining reveals the characteristics of nucleic acid and protein expression, and Raman spectroscopy reveals Raman peak shifts of nucleic acids, protein expression, and the lipid structure in lung cancer cells, laying a foundation for Raman spectroscopy to be used to set the substance peak of tumor cells.