A Lab-on-a-Chip For The Extraction Of Bacterial Nucleic Acids From Whole Human Blood

Matthias Hugle, Lara S. Siegel,Ole Behrmann,Frank T. Hufert,Gregory Dame,Gerald A. Urban

international conference on solid state sensors actuators and microsystems（2019）

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摘要

We present a lab-on-a-chip for the extraction of bacterial nucleic acids from whole human blood (WHB). Compared to classical methods, with extraction times between hours and days, the presented chip can lyre the bacteria suspended in WHB and extract their nucleic acids within three minutes. This is achieved by directly coupling thermal lysis to gel-electrophoresis on a single microfluidic platform.

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关键词

mRNA extraction,lab-on-a-chip,bacteria,whole human blood,sepsis

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