Gankyrin promotes osteosarcoma tumorigenesis by forming a positive feedback loop with YAP

•Gankyrin is upregulated in osteosarcoma (OS) and predicts OS progression and poor prognosis.•Gankyrin establishes a positive feedback loop to regulate yes-associated protein (YAP) signaling in OS cells.•Gankyrin interacts with YAP to promote OS cell growth in vitro.•Gankyrin promotes OS tumor growth and regulates YAP levels in vivo.•There is a positive correlation between gankyrin and YAP expression in human OS tissues.