Improvement in the thermal stability of Mucor prainii-derived FAD-dependent glucose dehydrogenase via protein chimerization.

Yosuke Masakari,Chiaki Hara,Yasuko Araki,Keiko Gomi,Kotaro Ito

Enzyme and Microbial Technology（2020）

引用 11|浏览1

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摘要

•Mr144–297 was more heat resistant and stable than glucose oxidase from A. niger.•Mr144–297 had both high affinity and accurate substrate specificity for D-glucose.•Mutation sites in Mr144–297 improved thermal stability and productivity of E. coli.

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关键词

FAD-dependent,Glucose dehydrogenase,Chimeric enzymes,Thermal stability

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