NMR-based metabolomics analysis identifies discriminatory metabolic disturbances in tissue and biofluid samples for progressive prostate cancer.

•Metabolic alteration is more noticeable in tumor tissue than in biofluids of PCa.•Energy metabolism is reduced especially in tumor tissue during PCa progression.•Urinary choline metabolism is decreased during PCa progression.•Amino acid metabolism in biofluids reflects energy metabolism in tumor tissue.•CRPC patients have a decreased amino acid metabolism in serum.