Using Affinity Purification Coupled with Stable Isotope Labeling by Amino Acids in Cell Culture Quantitative Mass Spectrometry to Identify Novel Interactors/Substrates of Protein Arginine Methyltransferases.

•Altered PRMT expression or deregulation contributes to pathology of a number of diseases.•PRMTs have lower dissociation rates with their hypomethylated substrates.•PRMT6 inhibition with a small molecular inhibitor resulted in identification of known PRMT6 substrates.•This methodology can be used to identify potential new PRMT substrates which may be beneficial for therapeutic purpose.