STIM1 long and STIM1 gate differently TRPC1 during store-operated calcium entry.

•Ionic channels gated by the muscle specific STIM1L isoform are poorly characterized.•Current recordings on HEK cells showed that STIM1 and STIM1L activate Orai1 and TRPC1.•STIM1L is more prone to open TRPC1, while less efficient in Orai1 opening.•The stochiometry between STIM1/L-Orai1-TRPC1 is important for channel opening.•The larger STIM1L-induced SOCE compared to STIM1 is likely due to TRPC1 activation.