Comparative transcriptome analysis of genomic region deletion strain with enhanced l -tyrosine production in Saccharomyces cerevisiae

Objective To determine the effect of large genomic region deletion in a Saccharomyces cerevisiae strain on tyrosine yield and to identify new genetic modification targets through transcriptome analysis. Results TAL was used to produce p -coumaric acid ( p- CA) from tyrosine to quantity tyrosine yield. S. cerevisiae mutant strain NK14 with deletion of a 23.8 kb genomic region was identified to have p- CA production of 10.3 mg L − 1 , while the wild-type strain BY4741 had p- CA production of 1.06 mg L − 1 . Analysis of growth patterns and stress tolerance showed that the deletion did not affect the growth phenotype of NK14. Transcriptome analysis suggested that, compared to BY4741, genes related to glycolysis ( ENO2 , TKL1 ) and the tyrosine pathway ( ARO1, ARO2, ARO4, ARO7 , TYR1 ) were upregulated in NK14 at different levels. Besides genes related to the tyrosine biosynthetic pathway, amino acid transporters ( AVT6 , VBA5 , THI72 ) and transcription factor ( ARO80 ) also showed changes in transcription levels. Conclusions We developed a strain with improved tyrosine yield and identified new genetic modification candidates for tyrosine production.