The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7.

•Tyrosine-phosphorylated claudin-7 affected the TRPV4-modulated intestinal epithelial barrier.•Tyrosine phosphorylation of claudin-7 affected the protein expression of TRPV4.•Tyrosine-phosphorylated claudin-7 affected TRPV4-mediated calcium influx.