In Vitro Differentiation of CD4 + T Cell Effector and Regulatory Subsets.

In vitro differentiation of naïve CD4 T cells into effector and regulatory subsets offers a means to acquire large numbers of relatively homogeneous cell populations for experimentation. However, culture systems for T cell differentiation described in the literature vary widely in efficiency and complexity, limiting their comparison across studies. Here, we present a standardized and robust method for the isolation and in vitro differentiation of six CD4 T cell subsets from mouse naïve T cells.