TRIM32 triggers beta-catenin signaling through ubiquitylation of AXIN1 to promote inflammatory factor-induced apoptosis of rat nucleus pulposus cells

The dysregulation of ubiquitin ligase is the cause of many human diseases. Tripartite motif protein 32 (TRIM32) is an E3 ubiquitin ligase whose role in nucleus pulposus (NP) cell apoptosis is unclear. The expression of TRIM family protein and beta-catenin in 40 NP tissue samples was detected by RT-PCR. Interleukin (IL)-1 beta or tumor necrosis factor (TNF)-alpha was used to treat rat NP cells. Knockdown and overexpression of Trim32 were achieved using specific siRNA and recombinant plasmids. Western blotting, RT-PCR, and flow cytometry were used to assess the expression of TRIM32/beta-catenin and the apoptosis rate of NP cells. Coimmunoprecipitation was adopted to analyze the possible interactions between AXIN1 and TRIM32. In clinical samples, TRIM32 expression was of positive relevance with the expression of CTNNB1 (beta-catenin). In vitro, apoptosis of IL-1 beta- or TNF-alpha-treated rat NP cells was induced through upregulated Trim32 expression and activated beta-catenin signaling, whereas Trim32 siRNA and inhibition of beta-catenin reversed the induction effect of cytokines. Further studies indicated that TRIM32 activated the beta-catenin signaling pathway through ubiquitination of AXIN1, thereby regulating apoptosis. Collectively, this study reveals that TRIM32 promotes inflammatory factor-induced apoptosis of rat NP cells, in part by direct degradation of AXIN1 to trigger beta-catenin signaling.