TNFα-Signaling Modulates the Kinase Activity of Human Effector Treg and Regulates IL-17A Expression.

Maintenance of regulatory T cells CD4(+)CD25(high)FOXP3(+) (Treg) stability is vital for proper Treg function and controlling the immune equilibrium. Treg cells are heterogeneous and can reveal plasticity, exemplified by their potential to express IL-17A. TNF alpha-TNFR2 signaling controls IL-17A expression in conventional T cells via the anti-inflammatory ubiquitin-editing and kinase activity regulating enzyme TNFAIP3/A20 (tumor necrosis factor-alpha-induced protein 3). To obtain a molecular understanding of TNF alpha signaling on IL-17 expression in the human effector ((eff)Treg, CD25(high)CD45RA(-)) Treg subset, we here studied the kinome activity regulation by TNF alpha signaling. Using FACS-sorted naive ((naive)Treg, CD25(high)CD45RA(+)) and (eff)Treg subsets, we demonstrated a reciprocal relationship between TNF alpha and IL-17A expression; (eff)Treg (TNF alpha(low)/IL-17A(high)) and (naive)Treg (TNF alpha(high)/IL-17A(low)). In (eff)Treg, TNF alpha-TNFR2 signaling prevented IL-17A expression, whereas inhibition of TNF alpha signaling by clinically applied anti-TNF antibodies led to increased IL-17A expression. Inhibition of TNF alpha signaling led to reduced TNFAIP3 expression, which, by using siRNA inhibition of TNFAIP3, appeared causally linked to increased IL-17A expression in (eff)Treg. Kinome activity screening of CD3/CD28-activated (eff)Treg revealed that anti-TNF-mediated neutralization led to increased kinase activity. STRING association analysis revealed that the TNF suppression (eff)Treg kinase activity network was strongly associated with kinases involved in TCR, JAK, MAPK, and PKC pathway signaling. Small-molecule-based inhibition of TCR and JAK pathways prevented the IL-17 expression in (eff)Treg. Together, these findings stress the importance of TNF-TNFR2 in regulating the kinase architecture of antigen-activated (eff)Treg and controlling IL-17 expression of the human Treg. These findings might be relevant for optimizing anti-TNF-based therapy and may aid in preventing Treg plasticity in case of Treg-based cell therapy.