Alpha- and gamma-tocopherol levels in human semen and their potential functional implications.

Vitamin E is a potent antioxidant that has been considered involved in fertility, but studies have mostly focused on alpha-tocopherol. Our study aimed at measuring, by an isotope dilution gas chromatography-mass spectrometry method, alpha- and gamma-tocopherol concentration in human semen in a large and well-characterised population (134 men with different semen parameters and in varicocele patients), as well as their potential role in male fertility. We carried out freeze/thaw experiments in 15 samples with the two isomers in the cryoprotective medium. Moreover, our study included 10 subjects supplemented in vivo with alpha-tocopherol for 90 days. In seminal plasma, gamma-tocopherol concentration was significantly lower in the varicocele group than in the normozoospermic group. We observed that gamma-tocopherol, supplemented to cryopreservation medium, induced a higher post-thaw human sperm viability and motility than alpha-tocopherol. The results of in vivo alpha-tocopherol supplementation showed a decrease in gamma-tocopherol concentration with increasing alpha-tocopherol level in blood. This is the first report related to gamma-tocopherol distribution in human semen analysed by gas chromatography-mass spectrometry. gamma-tocopherol would not seem to be related to semen parameters but to cellular oxidative condition. This tocopherol may contribute to human health in a yet unexplored way.