Effects of Extraction Factors on Total Phenolic Compounds and Antioxidant Activity in Mulberry Leaves

The objective of this study is to examine effects of ultrasonic-assisted extraction on bioactive compounds of mulberry leaves in compared with the conventional extraction method. The mulberry leaves used for this research were cleaned and dried by hot air drying technique at 60°C for 5 hours. The ethanol concentration of 50% and 80% with ethanol soaking time of 60 minutes and extraction time of ultrasonic treatments at 20, 30 and 40 minutes were used. The experimental results showed that the ultrasonic-assisted extraction provided highest total amout of phenolic content and DPPH scavenging activity compared with the controlled samples (p≤0.05). In addition, increasing extraction time could further increase total phenolic content and DPPH scavenging activity; with 50% ethanol and extraction time of 40 minutes provided the highest total phenolic content and DPPH scavenging activity under the tests.