Impact of Holder pasteurization on essential elements from human donor milk: Total contents and protein-binding profiles

•Speciation studies of Cu, Fe, Se and Zn in human milk were conducted by SEC-ICP-MS.•Holder pasteurization did not influence Cu, Fe, Se and Zn contents in human milk.•Protein profiles of human donor milk were affected by Holder pasteurization.•Holder pasteurization caused a little interconversion of Cu, Fe, Se and Zn species.•Apo-proteins rather than holo-proteins are affected by Holder pasteurization.