P1588 micro rna expression profiling may predict cardiac remodeling after stemi

Abstract Background MicroRNAs have been recognised as important modulators of cardiovascular function. However, their profiling in ischemic heart disease and contribution to cardiac remodeling has not been defined. Methods We examined 40 patients with STEMI and 20 healthy controls. MicroRNA expression profiling was carried out within 48 hours of the index ischemic event, measuring the expression of microRNA-144,-150,-499 (cardioprotective action), -21, and -208 (remodeling stimuli). In addition, every patient was evaluated by echocardiography, which was repeated after a 2-year follow-up period. Results Left-ventricular end-systolic volume (LVESV) and left-ventricular end-diastolic volume (LVEDV) decreased (from 57.7± 6.3 to 48.6± 5.2, p < 0.05 and from 102.1± 7.1 to 85.9 ± 5.7, p < 0.05, respectively) while the ratio of early mitral inflow velocity to mitral annular early diastolic velocity (E/e’) did not change (from 9.32± 0.6 to 9.56± 0.8, p= NS). Mir-208 and -499 expression within 48 hours of STEMI (1.91± 0.43/U6sn and 1.7± 0.48/U6sn respectively) were significantly positively correlated with a reduction in LVESV, LVEDV, and E/e’. In specific, mir-208 expression was associated with an absolute (r= -0.41, p < 0.05) and a percent reduction (r=-0.45, p= 0.03) in LVEDV and an absolute reduction in E/E’ (r= 0.46, p < 0.05), while mir-499 was associated with an absolute (r= -0.4, p < 0.05) and percent reduction (p= -0.38, p < 0.05) in LVESV. Mir-499 median value (1.78[1.292.01]) predicted reverse remodelling (LVESV reduction by >15%) with satisfactory specificity (0.82). Conclusion Mir- 208 and -499 expression may contribute to cardiac remodeling after STEMI, while mir-499 could be used as a prognostic marker of reverse remodeling.