Liquid chromatography above 20,000 PSI

TrAC Trends in Analytical Chemistry(2020)

引用 21|浏览11
暂无评分
摘要
Continued improvements in HPLC have led to faster and more efficient separations than previously possible. One important aspect of these improvements has been the increase in instrument operating pressure and the advent of ultrahigh pressure LC (UHPLC). Commercial instrumentation is now capable of up to ~20 kpsi, allowing fast and efficient separations with 5–15 cm columns packed with sub-2 μm particles. Home-built instruments have demonstrated the benefits of even further increases in instrument pressure. The focus of this review is on recent advancements and applications in liquid chromatography above 20 kpsi. We outline the theory and advantages of higher pressure and discuss instrument hardware and design capable of withstanding 20 kpsi or greater. We also overview column packing procedures and stationary phase considerations for HPLC above 20 kpsi, and lastly highlight a few recent applications of ultrahigh pressure instruments for the analysis of complex mixtures.
更多
查看译文
关键词
Ultrahigh pressure LC,Column packing,Small particles,Long columns,Omics
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要