Muscone relieves inflammatory pain by inhibiting microglial activation-mediated inflammatory response via abrogation of the NOX4/JAK2-STAT3 pathway and NLRP3 inflammasome.

•Muscone inhibited the releases of IL-6, TNF-α, and IL-1β in LPS-induced BV2.•Muscone blocked LPS-triggered inflammatory via suppressing the NOX4 expression.•NOX4-mediated activation of JAK2-STAT3 pathway is involved in muscone-inhibited inflammation.•Administration with muscone mitigated CFA-induced pain hypersensitivity.•Muscone restrained CFA-induced inflammation and microglia cell activation.