Restoration of Out-of-Focus Fluorescence Microscopy Images Using Learning-Based Depth-Variant Deconvolution

Image quality is degraded in the out-of-focus region because of the depth-variant (DV) point spread function (DV-PSF) of a fluorescence microscope. Either non-blind or blind deconvolution for restoration results in limited improvement. In this work, we propose a two-step learning-based DV deconvolution (LB-DVD) to restore the out-of-focus image. In the first step, DV-PSF is predicted by a defocus ...