ANNALS EXPRESS: Plasma viscosity: Evaluation of a new measuring method using microfluidic chip technology (microVisc) for clinical use and determination of a new reference range.
ANNALS OF CLINICAL BIOCHEMISTRY(2020)
摘要
Background Plasma viscosity is an important biomarker both in diagnostics and treatment monitoring of plasma cell dyscrasias and other disorders with hyperviscosity syndrome as a clinical manifestation. Here, we investigate the performance of a new microfluidic-based viscometer for clinical use, establish a new reference range to be used with this instrument and determine the importance of sampling temperature. Methods The microVisc (TM) viscometer was evaluated for within-run and between-run imprecision and bias using standardized reference material (Paragon controls) and Seronorm (TM) control material. The reference range was established for the adult population using EDTA-plasma from 120 healthy blood donors. Sampling temperature was investigated by drawing and transporting blood at room temperature and 37 degrees C and comparing the viscosity between the two sampling methods. Results The microfluidic-based viscometer performed well, and imprecision was comparable to ReoRox (R) G2 free oscillation rheometer. A new reference range for the adult Danish population was established as 1.2-1.5 mPa s at 37 degrees C. Furthermore, sampling temperature at room temperature and 37 degrees C was investigated, and there was no difference in results obtained. Conclusions MicroVisc (TM) is suitable for measuring plasma viscosity in a clinical setting and results can be evaluated using the established reference range. Blood sampling for viscosity analysis can be performed as a standard procedure at room temperature.
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关键词
Viscometer,rheology,plasma viscosity,microfluidic,reference interval
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