Normal phase HPLC method for combined separation of both polar and neutral lipid classes with application to lipid metabolic flux.

Journal of Chromatography B（2020）

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摘要

•Normal phase HPLC method to separate polar and neutral lipid classes in a single run.•Three variations of the method based on the nature of lipid mixture.•Application to lipid extracts from animal, microbial and plant origins.•Application to in vivo metabolic flux with 14C metabolic tracers.

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关键词

Phospholipids,Galactolipids,Triglycerides,Hydroxy fatty acids,Radioactivity,Metabolic flux

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