Applications Of Click Chemistry Reaction For Proteomics Analysis

Cu (I) -catalyzed azide-alkyne specific reaction, popularly known as the "click chemistry reaction", is defined as an important molecular connection method, which can realize simple and efficient connection between specific small molecule groups at the molecular level. Because of its advantages such as high efficiency, high selectivity, mild conditions and few side reactions, click chemistry reaction has been widely used in proteomics analysis, including the identification of newly synthesized proteins and the identification of various post-translational modified proteins. Therefore, in-depth study of the reaction mechanism of click chemistry reaction in proteomics is very important for understanding the structure and function of proteins. The goal of this review is to highlight the progress of several important applications of click chemistry in proteomics analysis in recent years, and discuss the future trends of the click chemistry in proteomics.