Ad Hoc Laboratory -Based Surveillance of SARS-CoV-2 by Real-Time RT-PCR Using Minipools of RNA Prepared from Routine Respiratory Samples

Anna M. Eis-Huebinger,Mario Hoenemann,Juergen J. Wenzel,Annemarie Berger,Marek Widera,Barbara Schmidt,Souhaib Aldabbagh,Benjamin Marx,Hendrik Streeck,Sandra Ciesek,Uwe G. Liebert,Daniela Huzly,Hartmut Hengel,Marcus Panning

JOURNAL OF CLINICAL VIROLOGY（2020）

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摘要

•A laboratory-based surveillance tool for SARS-CoV-2 was established.•It consists of minipool testing of nucleic acid preparations.•Limit of detection was 48 copies per reaction (95 % confidence interval: 33–184).•A protocol was distributed among five German university hospitals.•The approach proved its principle and one COVID-19 case was detected in 70 minipools.

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关键词

SARS-CoV-2,RT-PCR,Minipools,Surveillance,Laboratory

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