Quantification of chitooligosaccharides by FACE method: Determination of combinatory effects of mouse chitinases.

Masahiro Kimura,Takatoshi Umeyama,Satoshi Wakita,Kazuaki Okawa,Masayoshi Sakaguchi,Vaclav Matoska,Peter O Bauer,Fumitaka Oyama

MethodsX（2020）

引用 3|浏览23

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摘要

•FACE is a simple, qualitative and quantitative method.•The standard curve warrants quantification of chitooligosaccharides of up to 10 nmol regardless on used buffer system.•Our improved FACE method enable us to quantify chitooligosaccharides produced by chitinases at pH 2.0–8.0.•Determination of the combinatory effects of the Chit1 and AMCase using the FACE method.

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Improved FACE method

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