Performance of the Check-Direct ESBL Screen for BD MAX TM for detection of asymptomatic fecal carriage of ESBL producing Escherichia coli and Klebsiella pneumoniae.

Journal of Global Antimicrobial Resistance(2020)

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摘要
•The performance of direct quantitative PCR based methods was evaluated in the detection of faecal ESBL carriage.•Culture-based method was used as a gold standard.•Whole genome sequencing (WGS) was used to confirm the ESBL genes.•The frequency of qPCR positive samples was 12.3 % and with culture based method 6.3 %, respectively.•Culture negative but qPCR positive discrepancy was observed in 6.9 % of the tested samples
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关键词
Antimicrobial resistance,ESBL,Diagnostics,Escherichia coli,Klebsiella pneumoniae,Whole-genome sequencing
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