pMOX: a new powerful promoter for recombinant protein production in yeast Pichia pastoris

Mohsen Mombeni,Sareh Arjmand,Seyed Omid Ranaei Siadat,Houshang Alizadeh,Alireza Abbasi

Enzyme and Microbial Technology（2020）

引用 13|浏览3

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摘要

•pMOX from H. polymorpha was explored for the heterologous expression of proteins in P. pastoris.•pMOX showed a different pattern of induction in P. pastoris compared with its native host.•Gene dosage and protease deficient-trait affect genes expression under the control of pMOX.•Methanol-free pMOX is introduced as a satisfactory alternative for pAOX1 in P. pastoris.

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关键词

Promoter,Recombinant protein,Pichia pastoris,Cellulase,Gene dosage,Strain

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