Effects of electroacupuncture combined with olfactory ensheathing cell transplantation on growth associated protein GAP-43 levels in the injured spinal cord region

Purpose: The study of electro-acupuncture combined with olfactory ensheathing cells ( OECs ) transplantation for treatment of rats with spinal cord injury ( SCI ) of spinal motor function recovery, pathological change and the relationship between them. In order to determine the effect of the combination of the two, and whether a single processing factors can make the axonal regeneration and the regeneration of axons have oriented sex effect, so that the spinal cord function recovered to a certain extent, electroacupuncture and olfactory ensheathing cells in repair of spinal cord injury process produces a synergistic effect and its mechanism of action. Methods: In the experiment,72 adult SD rats with contusion and transection method causes the T9 model of spinal cord injury,72 adult female Wistar rats were randomly divided into four groups,with 18 in the individual group.A:Model group;B:OECs group;C:electro-acupuncture group;D:electro-acupuncture and OECs group. After building the model for 1 days,1W,2W,3W,5W,9W,applications of the modified BBB scoring method evaluation before and after treatment of injury of rat nerve function to improve the situation; in molding after 2W, 3W, 5W, 9W in each group in a random sample of3, application of chloral hydrate intraperitoneal injection anesthesia death, cardiac lavage after removing the damaged spinal cord tissue, immunohistochemical determination of GAP-43. Results: The first, to model after 1D,1W,2W,3W,5W,9W. BBB score, molding 1D after each score is 0points,1W groups of rat hindlimb has recovered, but the score is low, the group had no difference (P>0.05);2W B group, C group, D group was higher than that of A group were significantly different (P<0.05);3W,5W,9W score in group D was higher than that in group B, group C had significant difference (P<0.05). The second ,in the 1W,2W,3W,5W growth to protein GAP-43 immunohistochemical assay,1W group content were increased but no significant difference;2W,3W,5W B group, C group, D group was higher than that of A group were significantly different (P < 0.05), D group higher than that of B group, C group had significant difference (P < 0.01).The third in B group, C group, D group than in A group light complications. Conclusions: The BBB scoreandGAP-43detection, inference of electro-acupuncture combined with OECs transplantation in treatment of spinal cord injury in rats at the axon growth there is a synergy, spinal nerve function is greatly improved.View Large Image Figure ViewerDownload Hi-res image Download (PPT)View Large Image Figure ViewerDownload Hi-res image Download (PPT)