Temperature-dependent Mitochondrial-Nuclear Epistasis.

Figure 1.: Agarose gel electrophoresis genotype analysis of some of the AF16-HK105 AI-RIL at the cb-m127 indel marker. Six AI-RIL genomic DNA templates (FV###) and control templates (positive: AF16 and HK105 genomic DNA samples; negative: water) were PCR amplified and then electrophoresed. For quality control purposes, the other six AIRIL templates shown were amplified in duplicate. The AF16 control amplicon is ~600 bp in size; the HK105 control amplicon is ~900 bp in size, as predicted by (Koboldt et al. 2010). M: 1 kb ladder (Promega, G5711).