Retina Microglial Activation And Functional Deficits In An Impact Concussion Mouse Model.Of Traumatic Brain Injury (Tbi)

Purpose: Traumatic Brain Injury (TBI) induces brain inflammation characterized by reactive microgliosis, infiltration of peripheral monocytes, and pro-and anti-inflammatory molecules. These responses can either facilitate recovery or trigger sequelae. Activation and interaction of specific immune cells and phenotypes are determinative of temporal pacing and outcome of brain recovery after concussive head injury.Methods: We utilized Ccr2RFP/Cx3cr1GFP mice (Jackson Laboratory) to enable immune cell visualization by class (microglia, monocyte), origin (peripheral, brain, retina), morphology, and location. We used a closed-head impact injury mouse model that recapitulates key features of human concussion. Experimental injury was conducted without anesthesia (approved IACUC protocol) to enable quantitative neurological assessment (Boston University Concussion Scale, BUCS). We evaluated histopathology …