Optimization of 2-DE and multiplexed detection of O-GlcNAcome, phosphoproteome and whole proteome protocol of synapse-associated proteins within the rat sensorimotor cortex.

•Optimization of separating conditions for 2D-gel electrophoresis of synapse associated proteins within the cerebral cortex.•Optimization of a multiplexed proteomic strategy to detect O-GlcNAc proteins, phosphoproteins, and the whole proteome within the same 2-D gel.•Applicable on low amounts of sample material of rat brain tissue.