Tumor Arrests DN2 to DN3 Pro T Cell Transition and Promotes Its Conversion to Thymic Dendritic Cells by Reciprocally Regulating Notch1 and Ikaros Signaling.
FRONTIERS IN IMMUNOLOGY(2020)
摘要
Tumor progression in the host leads to severe impairment of intrathymic T-cell differentiation/maturation, leading to the paralysis of cellular anti-tumor immunity. Such suppression manifests the erosion of CD4(+)CD8(+)double-positive (DP) immature thymocytes and a gradual increase in CD4(-)CD8(-)double negative (DN) early T-cell progenitors. The impact of such changes on the T-cell progenitor pool in the context of cancer remains poorly investigated. Here, we show that tumor progression blocks the transition of Lin(-)Thy1.2(+)CD25(+)CD44(+)c-Kit(low)DN2b to Lin(-)Thy1.2(+)CD25(+)CD44(-)c-Kit(-)DN3 in T-cell maturation, instead leading to DN2-T-cell differentiation into dendritic cells (DC). We observed that thymic IL-10 expression is upregulated, particularly at cortico-medullary junctions (CMJ), under conditions of progressive disease, resulting in the termination of IL-10R(high)DN2-T-cell maturation due to dysregulated expression of Notch1 and its target, CCR7 (thus restricting these cells to the CMJ). Intrathymic differentiation of T-cell precursors in IL-10(-/-)mice andin vitrofetal thymic organ cultures revealed that IL-10 promotes the interaction between thymic stromal cells and Notch1(low)DN2-T cells, thus facilitating these DN2-T cells to differentiate toward CD45(+)CD11c(+)MHC-II(+)thymic DCs as a consequence of activating the Ikaros/IRF8 signaling axis. We conclude that a novel function of thymically-expressed IL-10 in the tumor-bearing host diverts T-cell differentiation toward a DC pathway, thus limiting the protective adaptive immune repertoire.
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关键词
thymus,T cell,IL-10,DN2b,DC,Cancer
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